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Add slowly 500 ml Base B
10x concentrate
Na L-Ascorbate = 11
NaCl = 152
Pour 100 ml PBS (10x) into a flask
PBS - Phosphate Buffered Saline (500 ml) - 10x concentrate Add slowly 500 ml BasePhosphate Buffered Saline (PBS) is used as isotonic buffer solution in biological research. Product information 10x concentrate autoclaved Instructions for use: 10x concentrate: For 1000 ml PBS Solution: Pour 100 ml PBS (10x) into a flask Add deionized water to a final volume of 1000 ml Adjust to pH 7. 4 Use immediately once opened. Store at 4 8 C Ional composition of NFR Solution NaCl = 137 mM Na2HPO4 = 10 mM KCl = 2,7 mM KH2PO4 = 2 mM
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