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Then a biotinylated detection antibody specific for Rat IGFBP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then a biotinylated detection antibody specific for Human cTnT/TNNT2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Only those wells that contain Human COMP
a horseradish peroxidase conjugated mouse anti-Rat Beta-2 microglobulin Antibody is added to the wells and binds to Beta-2 microglobulin in the sample
and the color intensity is directly proportional to the amount of Human IL-7 present
Bovine AFP (Alpha-fetoprotein) ELISA Kit TFI Then a biotinylated detection antibodyThe Bovine (AFP) Alpha fetoprotein ELISA Kit measures Alpha fetoprotein in Bovine samples. The plate has been pre coated with Bovine AFP antibody. AFP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine AFP Antibody is added and binds to AFP in the sample. Then Streptavidin HRP is added and binds to the Biotinylated AFP antibody. After incubation unbound Streptavidin HRP is washed away during a
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