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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL2
and the Human PG standard plate wells that pre-coated using protein-related techniques are provided separately
The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm
The concentration of Human PGI in the samples is then determined by comparing the OD of the samples to the standard curve
mixing with PCR-amplified fragments that are completely homologous to the vector (15-40 bp) and reacting at 37C
Human IFN-gamma (Interferon Gamma) ELISA Kit CEA The microtiter plate provided inThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human IFN Gamma. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human IFN Gamma. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is
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