GAPDH (HRP Conjugated) Mouse mAb IFNB Samples (or Standards) and Horseradish

Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgA are added to the micro ELISA plate wells

The Human NOX5 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 5) ELISA kit uses the Sandwich-ELISA principle

coli expression system and the target gene encoding His25-Ser153 is expressed

Then a biotinylated detection antibody specific for Human IL-23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Only those wells that contain Rat GROBeta/CXCL1

GAPDH (HRP Conjugated) Mouse mAb IFNB Samples (or Standards) and HorseradishGlyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.