The concentration of Human CD83 in the samples is then determined by comparing the OD of the samples to the standard curve
TRIM54 present in the sample is added and binds to antibodies coated on the wells
The concentration of HDAC2 in the sample was calculated by drawing a standard curve
Calculate the concentration of TGF-β1 in the sample by plotting standard curve
Then a biotinylated detection antibody specific for Rat ARG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Rat BDH1 (D-beta-hydroxybutyRate dehydrogenase, mitochondrial) ELISA Kit TNF receptor superfamily member The concentration of Human CD83The Rat BDH1 (D beta hydroxybutyRate dehydrogenase, mitochondrial) ELISA Kit measures Bdh1 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Bdh1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components.