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Nmur1 present in the sample is added and binds to antibodies coated on the wells
Then a biotinylated detection antibody specific for Human IgM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Rat SOD1 in tested samples can be calculated by comparing the OD of the samples to the standard curve
Upon apoptotic stimulation
The concentration of Rat NGFIB in the samples is then determined by comparing the OD of the samples to the standard curve
Mouse SERPINB8 (Serpin B8) ELISA Kit Packaging:96 wells Nmur1 present in the sampleThe Mouse (SERPINB8) Serpin B8 ELISA Kit measures Serpin B8 in Mouse samples. The plate has been pre coated with Mouse SERPINB8 antibody. SERPINB8 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SERPINB8 Antibody is added and binds to SERPINB8 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SERPINB8 antibody. After incubation unbound Streptavidin HRP is washed away
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