Canine HAMP (Hepcidin) ELISA Kit Packaging:96 wells Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Human CA6

The plate has been pre-coated with Human MMP-7 antibody

Then Streptavidin-HRP is added and binds to the Biotinylated MYL4 antibody

The concentration of Rat MIP1a in the samples is then determined by comparing the OD of the samples to the standard curve

Then a biotinylated detection antibody specific for Human NSE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Canine HAMP (Hepcidin) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Canine HAMP (Hepcidin) ELISA Kit measures HAMP in Canine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti HAMP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.