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Then Streptavidin-HRP is added and binds to the Biotinylated BAD antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VIM
only those wells that contain Human LAMa4
The concentration of Rat S100A4 in the samples is then determined by comparing the OD of the samples to the standard curve
The plate has been pre-coated with Human GLRX2 antibody
Human FSCN1 (Fascin) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Human (FSCN1) Fascin ELISA Kit measures Fascin in samples. The plate has been pre coated with Human FSCN1 antibody. FSCN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human FSCN1 Antibody is added and binds to FSCN1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated FSCN1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
