Canine HCRT (Orexin) ELISA Kit CD144 a horseradish peroxidase conjugated mouse

a horseradish peroxidase conjugated mouse anti-Human CD19 Antibody is added to the wells and binds to CD19 in the sample

The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 31 nm

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ASGR2

The concentration of Human CD200 in the samples is then determined by comparing the OD of the samples to the standard curve

the intensity of color is proportional to the concentration of hGH in the samples

Canine HCRT (Orexin) ELISA Kit CD144 a horseradish peroxidase conjugated mouseThe Canine (HCRT) Orexin ELISA Kit measures Orexin in Canine samples. The plate has been pre coated with Canine HCRT antibody. HCRT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine HCRT Antibody is added and binds to HCRT in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HCRT antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.