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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NLRP3
Calculate the concentration of HSP72 in the sample by plotting standard curve
Calculate the concentration of CD300LB in the sample by plotting standard curve
Substrate solution is then added and color develops in proportion to the amount of Mouse SEMA3G
The plate has been pre-coated with Chicken Ep-CAM antibody
Human IHH (Indian hedgehog protein) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human IHH (Indian hedgehog protein) ELISA Kit measures IHH in Human Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IHH antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IHH bound to
