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Substrate solution is then added and color develops in proportion to the amount of Human SMAD3
it binds with SULF2 bound to precoated antibody
Then a biotinylated detection antibody specific for Mouse TFF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
And then biotinylated Mouse CHRM2 Antibody is added and binds to CHRM2 in the sample
Then a biotinylated detection antibody specific for Human GTF2F1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Rabbit PC4 (Positive Cofactor 4) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Rabbit PC4 (Positive Cofactor 4) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rabbit PC4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit PC4 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro
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