Rat HSL (Hormone Sensitive Lipase) ELISA Kit Packaging:96 wells the TMB substrate (enzyme substrate)

the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue colored product that is directly proportional to the amount of leptin present

Unbound streptavidin HRP conjugate is removed by a washing step

Substrate solution is then added and color develops in proportion to the amount of Mouse TBC1D1

Substrate solution is then added and color develops in proportion to the amount of Human ATG3

You can calculate the concentration of Mouse TSH in the samples by comparing the OD of the samples to the standard curve

Rat HSL (Hormone Sensitive Lipase) ELISA Kit Packaging:96 wells the TMB substrate (enzyme substrate)The Rat (HSL) Hormone Sensitive Lipase ELISA Kit measures Hormone Sensitive Lipase in Rat samples. The plate has been pre coated with Rat LIPE antibody. LIPE present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat LIPE Antibody is added and binds to LIPE in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LIPE antibody. After incubation unbound Streptavidin HRP is washed away during