Human ET-2 (Endothelin 2) ELISA Kit BRK-3 Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VEGFA

overexpressed protein from wild-type protein expressed by the host organism

The OD value is proportional to the concentration of Rat CGRP1

And then biotinylated Human ARHGEF5 Antibody is added and binds to ARHGEF5 in the sample

The OD value is proportional to the concentration of Human DKK1

Human ET-2 (Endothelin 2) ELISA Kit BRK-3 Standards or samples are addedThe Human (ET 2) Endothelin 2 ELISA Kit measures Endothelin 2 in samples. The plate has been pre coated with Human ET 2 antibody. ET 2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ET 2 Antibody is added and binds to ET 2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ET 2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.