Human CTSH (Cathepsin H) ELISA Kit Corticoliberin Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Human IL-17D

Then a biotinylated detection antibody specific for Human Bcl-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

SLC17A6 present in the sample is added and binds to antibodies coated on the wells

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PRPH

The plate has been pre-coated with Human TPMT antibody

Human CTSH (Cathepsin H) ELISA Kit Corticoliberin Substrate solution is then addedThe Human CTSH (Cathepsin H) ELISA Kit measures CTSH in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CTSH antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.