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And then biotinylated Human AMTN Antibody is added and binds to AMTN in the sample
Then a biotinylated detection antibody specific for Mouse MBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The plate has been pre-coated with Human CHST14 antibody
Substrate solution is then added and color develops in proportion to the amount of Mouse CCL2
The concentration of Human PGLYRP2 in the samples is then determined by comparing the OD of the samples to the standard curve
Human H3cit-NE (neutrophil extracellular trap) ELISA Kit HCHOLA3 And then biotinylated Human AMTN