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coli expression system and the target gene encoding Val77-Leu233 is expressed with a 6His tag at the C-terminus
The plate has been pre-coated with Human LRAT antibody
The plate has been pre-coated with Human SPAM1 antibody
Substrate solution is then added and color develops in proportion to the amount of Human PNMT
Then Streptavidin-HRP is added and binds to the Biotinylated CSF3 antibody
Mouse ALCAM (CD166 antigen) ELISA Kit HUAT coli expression system and theThe Mouse ALCAM (CD166 antigen) ELISA Kit measures ALCAM in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Alcam antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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