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Substrate solution is then added and color develops in proportion to the amount of Human SFTPB
Then Streptavidin-HRP is added and binds to the Biotinylated KRT77 antibody
only those wells that contain Human GZMH
Then Streptavidin-HRP is added and binds to the Biotinylated HEMGN antibody
The concentration of Human EFNA5 in the samples is then determined by comparing the OD of the samples to the standard curve
Rat PENK (Proenkephalin-A) ELISA Kit PBF2 Substrate solution is then addedThe Rat PENK (Proenkephalin A) ELISA Kit measures Penk in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Penk antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
