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The concentration of Human MAP1LC3B in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Mouse HDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-colored product that is inversely proportional to the amount of pregnenolone present
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL5a2
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NFE2L2
Rat ITGb3 (Integrin Beta 3) ELISA Kit (Small Sample) HME The concentration of Human MAP1LC3BThe Rat ITGb3 (Integrin Beta 3) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat ITGb3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat ITGb3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added,
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